therapeutic angiogenesis promotes efficacy of human umbilical cord matrix stem cell transplantation in cardiac repair

Authors

kaveh moradi department of anatomy, medical school, tehran university of medical sciences, tehran, iran

mehdi abbasi department of anatomy, medical school, tehran university of medical sciences, tehran, iran

farid aboulhasani department of anatomy, medical school, tehran university of medical sciences, tehran, iran

niloufar abbasi faculty of medicine, tehran medical branch, islamic azad university, iran

abstract

objective(s):although previous studies have confirmed the beneficial effects of human umbilical cord matrix stem cell (hucm) transplantation post myocardial infarction (mi), but this stem cell resource has no potential to induce angiogenesis. in order to achieve the process of angiogenesis and cardiomyocyte regeneration, two required factors for cardiac repair agents were examined namely; hucm and vegf on an infarcted heart. the main objective of this research is to investigate the combinatory effect of dhucm and vegf transplantation on an infarcted heart. materials and methods:45 min of ligating the left anterior descending coronary artery, the mi-induced animals received 50 μl pbs, 5 μg vegf, 5×106 hucm cells alone, combined with 5 μg vegf and 5×106 differentiated hucm cells alone or combined with 5 μg vegf through intramyocardial injection. mi group, without hucm and vegf served as the control group. left ventricular function and angiogenesis were also evaluated. results: after eight weeks post mi, there were significant rise in left ventricular ejection farction in dhucm+vegf group compared to the other treated and non-treated groups (p<0.05). fibrosis tissue was markedly lower in the dhucm+vegf and hucm+vegf groups compared to the other treated and non-treated groups (p<0.05). despite these benefits, vascular density in dhucm+vegf group was not markedly different compared to vegf and hucm+vegf groups. the transplanted hucm and dhucm cells survived and migrated to the infarcted area. conclusion: our findings demonstrated that the dhucm cells transplantation combined with vegf were more efficient on an infarcted heart.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Therapeutic angiogenesis promotes efficacy of human umbilical cord matrix stem cell transplantation in cardiac repair

Objective(s):Although previous studies have confirmed the beneficial effects of human umbilical cord matrix stem cell (hUCM) transplantation post myocardial infarction (MI), but this stem cell resource has no potential to induce angiogenesis. In order to achieve the process of angiogenesis and cardiomyocyte regeneration, two required factors for cardiac repair agents were examined namely; hUCM ...

full text

Therapeutic angiogenesis promotes efficacy of human umbilical cord matrix stem cell transplantation in cardiac repair

OBJECTIVES Although previous studies have confirmed the beneficial effects of human umbilical cord matrix stem cell (hUCM) transplantation post myocardial infarction (MI), but this stem cell resource has no potential to induce angiogenesis. In order to achieve the process of angiogenesis and cardiomyocyte regeneration, two required factors for cardiac repair agents were examined namely; hUCM an...

full text

Hypoxia Preconditioning Promotes Survival And Clonogenic Capacity Of Human Umbilical Cord Blood Mesenchymal Stem Cells

Background: In recent decade, human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) provide enormous potential for appropriate cell therapy, but they have limited growth potential and cease to proliferate due to cellular senescence, so providing a strategy for increasing the stem cell survival is necessary.&nbsp; Methods: In this investigation, MSCs characterized by flow cytome...

full text

P 82: The Transplantation of Human Umbilical Cord Mesenchymal Stem Cells in Neonatal Strokes

Brain injuries that caused by strokes (result of intra partum ischemia) are a frequent cause of prenatal mortality and morbidity with limited therapeutic options. Transplanting human mesenchymal stem cells (hmscs) indicates improvement in hypoxic Ischemic brain injury (HIBD) by secretion growth factor stimulating repair processes (Hmscs) known as multi potent cells which isolated from bone marr...

full text

In Vivo Tracing of Human Umbilical Cord Matrix Stem Cells Useing MRI

Purpose: Human umbilical cord matrix (UCM) (Wharton jelly) stem cells labeling are tracking by MRI. Materials and Methods: After 48 hours incubation with USPIO human umbilical cord matrix (UCM) stem cells were labeled with USPIO by the means of receptor-mediated endocytosis. Prussian blue staining and Atomic absorption spectroscopy were performed to identify and show the iron oxide nanoparticle...

full text

Human umbilical cord mesenchymal stem cell transplantation restores damaged ovaries

Ovarian injury because of chemotherapy can decrease the levels of sexual hormones and potentia generandi of patients, thereby greatly reducing quality of life. The goal of this study was to investigate which transplantation method for human umbilical cord mesenchymal stem cells (HUMSCs) can recover ovarian function that has been damaged by chemotherapy. A rat model of ovarian injury was establi...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۶، صفحات ۵۶۳-۵۷۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023